Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Detection of the OSMR on lung fibroblasts. Cells were stained with no antibody or with anti-OSMR followed by FITC-labelled goat anti-rabbit IgG. Immunostaining was then analysed by flow cytometry.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Staining, Immunostaining, Flow Cytometry

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Proliferation of human lung fibroblasts in response to OSM. Cells were exposed to OSM at concentrations ranging from 0.5 – 100 ng ml−1 for 24, 48 or 72 h. Proliferative effect was examined using an MTS assay (A) and confirmed by [3H]-TdR uptake (B). The proliferative effect of OSM was completely abrogated by incubation of cells with neutralizing antibodies against OSMR and gp 130. Results are means of four separate experiments performed in triplicate. *P<0.05 compared to cells in serum free conditions.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: MTS Assay, Incubation

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Cell counts of human lung fibroblasts after incubation with OSM

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Incubation

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Detection of cyclin D1 expression in lung fibroblasts. Immunohistochemical staining for cyclin D1 was performed on unstimulated cells (D) or cells exposed to OSM (2 ng ml−1) for 24 h (E). The specificity of the cyclin D1 antibody was confirmed by substituting the primary antibody with non-immune mouse IgG1 (F). Cells were also stained with DAPI in order to enumerate cell nuclei (A – C). Immunofluorescent images from 1 μm sections were obtained by scanning laser confocal microscopy at 40×magnification.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Expressing, Immunohistochemical staining, Staining, Confocal Microscopy

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Effects of the tyrosine kinase inhibitor (genestein) and p42/44 MAPK inhibitor (PD98059) on OSM-induced proliferation. Fibroblasts were seeded in 24 well plates and incubated with genestein (10 μM) or PD98059 (50 μM) in the presence or in the absence of 2 ng ml−1 OSM. At 24 and 48 h cell numbers were assessed using an MTS assay. Results are means of four separate experiments performed in triplicate. *P<0.05 versus cells exposed to OSM 2 ng ml−1. #P<0.05 compared to 24 h time point.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Incubation, MTS Assay

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Effects of the non-selective COX inhibitor indomethacin and the selective COX-2 inhibitor nimesulide on OSM-induced proliferation, COX expression and PGE2 release. (a) Cells were treated with indomethacin (5 μM) or the selective COX-2 inhibitor nimesulide (5 μM) prior to incubation with OSM (2 ng ml−1). Proliferative responses to OSM were not influenced by either drug at 24 or 48 h. Detection of COX-1 and COX-2 protein. Fibroblasts were incubated for either 6 or 24 h with OSM (2 ng ml−1) or IL-1β (2 ng ml−1) and expression of both COX-1 and COX-2 was determined by Western analysis (b). PGE2 release in response to OSM. PGE2 release was measured by EIA in supernatants taken from fibroblasts treated with OSM (2 ng ml−1) for 24 and 48 h (c). Results are shown as means of four separate experiments. *P<0.05 compared to cells in serum free medium.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Expressing, Incubation, Western Blot

Journal:

Article Title: Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts

doi: 10.1038/sj.bjp.0704769

Figure Lengend Snippet: Effect of OSM on human lung fibroblast pro-collagen production. Fibroblasts were grown to confluence and incubated with 10 or 100 ng ml−1 OSM for 48 h and the production of hyp measured by HPLC. Data are means of six independent experiments. *P<0.05 compared to cells in serum free medium.

Article Snippet: The normal diploid human foetal lung fibroblast cell line was obtained from American Type Culture Collection (ATCC, Rockville, MD, U.S.A) and was used between passages 11 and 24.

Techniques: Incubation